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April 15, 2010
Massively Multi-parameter Single Cell Analysis by Mass Cytometry
  • Scott D. Tanner, President  Learn More
  • Dmitry R. Bandura, Director  Learn More
  • Olga I. Ornatsky, Director  Learn More
  • Technologies that drive bio-discovery are progressively shifting toward highly parallel, high-throughput, and highly multiplexed approaches that are able to extract larger amounts of data from smaller samples at increasing cost effectiveness. DVS Sciences Inc. offers a novel solution for a broad spectrum of bio-analytical challenges. This webinar will cover 3 key topics:

    Beyond 30 Parameters in Single Cells with Mass Cytometry

    Technologies that drive bio-discovery are progressively shifting toward highly parallel, high-throughput, and highly multiplexed approaches that are able to extract larger amounts of data from smaller samples at increasing cost effectiveness. A novel solution for a broad spectrum of bio-analytical challenges is now available. The new technology takes advantage of the high resolution and sensitivity of Inductively Coupled Plasma Mass Spectrometry (ICP-MS), combined with the many available stable isotopes of elements of the periodic table, to simultaneously determine many proteins in samples through the quantification of stable isotope tags bound to a wide variety of bioaffinity molecules. The instrumental heart of this integrated massively multiparameter technology is the Mass Cytometer. The instrument “reads” a novel class of reagents based upon a metal-chelating polymer tag that is linked to the antibody probes. The high resolution of the mass cytometer allows simultaneous 30+ measurements over 4 orders of magnitude in individual cells without the need for compensation, provides state-of-the-art sensitivity, and offers the unique potential for absolute quantitation.

    Mass Cytometry: Equipment and Reagents. How does it work?

    Mass Cytometry is a new technology that brings the power, resolution, sensitivity and quantitative capabilities of atomic mass spectrometry to high throughput single cell analysis in order to address the challenges of multi-parameter, quantitative flow cytometry. Individual cells that have been immunologically stained with stable isotope tags are injected into the analytical instrument that “reads” the tag elements. Because there are many available stable isotopes, and the mass spectrometer provides exquisite resolution between detection channels, many parameters can be measured as easily as one. The mass cytometer itself is a specific configuration of an Inductively Coupled Plasma Time-of-Flight Mass Spectrometer, tailored for the analysis of up to 1000 cells per second for as many parameters as there are available stable isotope tags. Data acquisition, details of instrument’s design and software workflow will be discussed.

    Mass Cytometry Methods

    The basic principles of mass cytometry method development, handling of elemental tags, and linked immunological methods using as an example highly multiplex (over 20 antibodies) assays will be discussed. Examples involve cultured human leukemia cell lines, normal blood analysis, and patient samples. The results are compared to those of traditional fluorescence-based cytometric analysis.


    Researchers with interest in the Bio-Analytical Instrumentation and Reagents, multiplex cell analysis, and flow cytometry, including the following:

    Academic Researchers:  Proteomic and genomic researchers that investigate gene expression, protein interaction and protein reaction pathways, which contributes to the fundamental knowledge of living organisms in normal and diseased states.

    Diagnostic Cell Analysts:  Those that study protein signatures, either for a given cell within a matrix of many cells, or for a collection of cells in order to distinguish between healthy and cancerous cell populations, and develop personalized treatment plans.

    Pharmaceutical Researchers:  Who are interested in multiplex analysis of cells, in order to identify potential drug candidates or metabolic screens.

    Research Clinicians:  For identification of specific cell content that can point to the presence of specific disease states.


    Scott D. Tanner, DVS Sciences, Inc.

    Scott Tanner is the President and CEO, as well as visionary for the DVS technology platform. He has led DVS Sciences for the past 5 years, successfully raise $13.3M in non-dilutive funding through innovative business strategies, and bringing the company’s first lines of instrumentation and reagent products into the marketplace. Prior to co-founding DVS Sciences in 2004, Scott was Principal Scientist with MDS Sciex, the analytical technologies division of MDS Health Group. In his 25 years at Sciex Scott held roles of increasing importance. From 1985 through 2000 Scott led the R&D effort that developed the line of ELAN ICP-MS products – a multi-ten million dollar global franchise. The products remain prominent (dominant when Scott was with Sciex) worldwide today in their market segment.


    Dmitry R. Bandura, DVS Sciences, Inc. 

    Dmitry Bandura is Director, Instrumentation development. He is a co-founder of DVS, and has been responsible for the development of system architecture and engineering development. Dmitry also has experience at MDS Sciex, where he took over responsibility for the R&D and market support efforts in atomic mass spectrometry when Scott was promoted to Principal Scientist. Prior to Sciex, Dmitry was fundamental to the development of the only commercial ICP-TOF-MS instrument for elemental analysis, a product that he developed with GBC Scientific Equipment in Australia.


    Olga I. Ornatsky, DVS Sciences, Inc.

    Olga Ornatsky is Director: R&D Biological applications and Product Application Lab, and a Principal of DVS. She has held senior scientist roles in several MDS companies. She has more than 15 years of experience in the commercial environment as a senior strategic product application developer and in providing advanced customer/collaborator support. Olga is a principal inventor on the methods and “apparatus therefore” patent applications.


    DVS Sciences, Inc. is an analytical equipment and reagents development company that produces and markets globally the CyTOF™ , a high throughput mass cytometer for individual cell analysis based on a novel elemental mass-spectrometry detection technology, and the MAXPAR™ system of novel reagents related to massively multi-parametric biological assays.

    For information about the recorded archive for this event contact Xtalks at (416) 977-6555 ext 371, or email register@xtalks.com

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